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MPP8 is essential for sustaining self-renewal of ground-state pluripotent stem cells.
Muller I, Moroni AS, Shlyueva D, Sahadevan S, Schoof EM, Radzisheuskaya A, Hojfeldt JW, Tatar T, Koche RP, Huang C, Helin K
Nat Commun. 2021 May 24;12(1):3034. doi: 10.1038/s41467-021-23308-4.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
175036pRepair-MPP8_mAID-P2A-BFPHomology Directed Repair construct for C-terminal tagging of mMPP8 with mAID using CRISPR/Cas9
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175037pL-U6-sgRNA-SFFV-Puro-P2A-EGFPLentiviral delivery of sgRNA. Expresses PuroR and eGFP from SFFV promoter.
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175038pPB-MPP8_Flag2-BlastMammalian expression of full length mMPP8 with C-terminal Flag2 tag
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175039pPB-MPP8-112–858_Flag2-BlastMammalian expression of mMPP8 (E112 to Q858) with C-terminal Flag2 tag
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175040pPB-MPP8-1–729_Flag2-BlastMammalian expression of mMPP8 (M1 to E729) with C-terminal Flag2 tag
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175041pPB-MPP8-∆ARD_Flag2-BlastMammalian expression of mMPP8 (deletion from D570 to K718) with C-terminal Flag2 tag
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Antibodies from Article