pPBT-peRNA_GG-Puro
(Plasmid #173220)

• Purpose: piggyBac transposon containing hU6 promotor and pegRNA cloning site
• Depositing Lab: Jacob Giehm Mikkelsen
• Publication: Wolff et al Front Genome Ed. 2021 Nov 12;3:786893. doi: 10.3389/fgeed.2021.786893. eCollection 2021.

Backbone

• Vector backbone: pPBT
• Total vector size (bp): 6945
• Vector type: Mammalian Expression ; piggyBac
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: pegRNA cloning cassette
• Alt name: pegRNA-GG-acceptor (BsmBI)
• Insert Size (bp): 1063
• Promoter: hU6

Cloning Information for Gene/Insert 1

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GAGGGCCTATTTCCCATGAT

Gene/Insert 2

• Gene/Insert name: PuroR
• Alt name: Puromycin
• Alt name: Puro
• Insert Size (bp): 600
• Promoter: EF1-a

Cloning Information for Gene/Insert 2

• Cloning method: Gibson Cloning
• 5′ sequencing primer: GGAGTTTCCCCACACTGAGT

Resource Information

• Supplemental Documents:
  • pPBT-pegRNA_GG-Puro.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry