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(Plasmid #173222)


Item Catalog # Description Quantity Price (USD)
Plasmid 173222 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Total vector size (bp) 14039
  • Vector type
    Mammalian Expression ; piggyBac
  • Selectable markers
    Puromycin ; PuroTK

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
  • Alt name
    Prime editor 2
  • Alt name
  • Species
  • Insert Size (bp)
  • Promoter CMV

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCAAAATGTCGTAACAACTCCGCCC
  • 3′ sequencing primer GCGGTCCGGATCGACGGTGT
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
  • Insert Size (bp)
  • Promoter P2A

Cloning Information for Gene/Insert 2

  • Cloning method Gibson Cloning
  • 5′ sequencing primer GCCGGAGATGTCGAAGAGAA
  • 3′ sequencing primer CTCAGACAATGCGATGCAAT
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    pegRNA cloning cassette
  • Alt name
    pegRNA-GG-acceptor (BsmBI)
  • Insert Size (bp)
  • Promoter hU6

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBT-PE2-PuroTK-pegRNA_GG was a gift from Jacob Giehm Mikkelsen (Addgene plasmid # 173222 ; ; RRID:Addgene_173222)
  • For your References section:

    piggyPrime: High-Efficacy Prime Editing in Human Cells Using piggyBac-Based DNA Transposition. Wolff JH, Haldrup J, Thomsen EA, Andersen S, Mikkelsen JG. Front Genome Ed. 2021 Nov 12;3:786893. doi: 10.3389/fgeed.2021.786893. eCollection 2021. 10.3389/fgeed.2021.786893 PubMed 34870275