pLenti-EF1a-CDK6-mutPAM-PGK-puro
(Plasmid
#187638)
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Purposesg resistant CDK6 ORF
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 187638 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLenti-EF1α-gate-3HA-PGK-Puromycin
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Backbone manufacturerGift from G. Lu
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameCDK6
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Alt nameCyclin Dependent Kinase 6
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SpeciesH. sapiens (human)
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MutationPAM site mutation
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Entrez GeneCDK6 (a.k.a. MCPH12, PLSTIRE)
Resource Information
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A portion of this plasmid was derived from a plasmid made byN. Persky, Broad Institute
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti-EF1a-CDK6-mutPAM-PGK-puro was a gift from William Kaelin (Addgene plasmid # 187638 ; http://n2t.net/addgene:187638 ; RRID:Addgene_187638) -
For your References section:
HIF-independent synthetic lethality between CDK4/6 inhibition and VHL loss across species. Nicholson HE, Tariq Z, Housden BE, Jennings RB, Stransky LA, Perrimon N, Signoretti S, Kaelin WG Jr. Sci Signal. 2019 Oct 1;12(601). pii: 12/601/eaay0482. doi: 10.1126/scisignal.aay0482. 10.1126/scisignal.aay0482 PubMed 31575731
