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HA-L-SA-2A-PuroR-GFP-KRASG12V-TRE-HA-R
(Plasmid #258167)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 258167 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Puro-iDEST
  • Backbone manufacturer
    Danwei Huangfu, Addgene plasmid #75336
  • Backbone size w/o insert (bp) 7051
  • Total vector size (bp) 6758
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GFP-KRAS-G12V
  • Alt name
    GFP-KRAS-G12V
  • Species
    H. sapiens (human); Aequorea victoria
  • Insert Size (bp)
    1353
  • Mutation
    KRAS G12V
  • GenBank ID
    U57608.1 NM_004985.5
  • Entrez Gene
    KRAS (a.k.a. 'C-K-RAS, C-K-RAS, CFC2, K-RAS2A, K-RAS2B, K-RAS4A, K-RAS4B, K-Ras, K-Ras 2, KI-RAS, KRAS1, KRAS2, NS, NS3, OES, RALD, RALD2, RASK2, c-Ki-ras, c-Ki-ras2)
  • Promoter TRE

Cloning Information

  • Cloning method Gateway Cloning
  • 5′ sequencing primer GGATTGGGAAGACAATAGCAGG
  • 3′ sequencing primer CGTCAGATCGCCTGGAGAAT
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HA-L-SA-2A-PuroR-GFP-KRASG12V-TRE-HA-R was a gift from Danwei Huangfu (Addgene plasmid # 258167 ; http://n2t.net/addgene:258167 ; RRID:Addgene_258167)

  • For your References section:

    Human pancreatic progenitor organoids define genetic and epigenetic barriers to early PDAC transformation. Zhang X, Wong W, Cho HS, Yang D, Dixon G, Luo R, Liu D, Torre D, Umeda S, Gonzalez F, Askan G, Yavas A, Damodaran JR, Rickert RW, Kappagantula R, Pan FC, Aveson VG, Grimont A, Fall WB, Wang X, Pulecio J, Kaplan SJ, Pan H, Peng XL, Yeh JJ, Leach SD, Iacobuzio-Donahue CA, Chandwani R, Leslie CS, Huangfu D. Dev Cell. 2026 May 19:S1534-5807(26)00159-0. doi: 10.1016/j.devcel.2026.04.012. 10.1016/j.devcel.2026.04.012 PubMed 42161274
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