PurposeConstitutive expression of a gRNA-tRNA array for macsGESTALT editing
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||178569||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 8327
- Total vector size (bp) 9423
Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
Growth Strain(s)NEB Stable
Gene/Insert name5 gRNA-tRNA array with U6 promoter
Insert Size (bp)1094
- Cloning method Gibson Cloning
- 5′ sequencing primer TRC-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please see associated article for information on this vector's use in the context of the macsGESTALT lineage recording system: https://doi.org/10.1016/j.ccell.2021.05.005
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUltra-U6-gRNAs1-5 was a gift from Christopher Lengner (Addgene plasmid # 178569 ; http://n2t.net/addgene:178569 ; RRID:Addgene_178569)
For your References section:Single-cell lineage tracing of metastatic cancer reveals selection of hybrid EMT states. Simeonov KP, Byrns CN, Clark ML, Norgard RJ, Martin B, Stanger BZ, Shendure J, McKenna A, Lengner CJ. Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 10.1016/j.ccell.2021.05.005. Epub 2021 Jun 10. 10.1016/j.ccell.2021.05.005 PubMed 34115987