pUltra-U6-gRNAs1-5
(Plasmid #178569)

• Purpose: Constitutive expression of a gRNA-tRNA array for macsGESTALT editing
• Depositing Lab: Christopher Lengner
• Publication: Simeonov et al Cancer Cell. 2021 Aug 9;39(8):1150-1162.e9. doi: 10.1016/j.ccell.2021.05.005. Epub 2021 Jun 10.

Backbone

• Vector backbone: pUltra
• Backbone size w/o insert (bp): 8327
• Total vector size (bp): 9423
• Vector type: Mammalian Expression, Lentiviral, CRISPR
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 5 gRNA-tRNA array with U6 promoter
• Insert Size (bp): 1094

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: TRC-F

Resource Information

• Supplemental Documents:
  • pdac-2020-pultra-u6-grnas1-5.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see associated article for information on this vector's use in the context of the macsGESTALT lineage recording system: https://doi.org/10.1016/j.ccell.2021.05.005