Purpose(Empty Backbone) Expresses Cpf1 customizable sgRNA from U6 promoter and puromycin resistance from EF-1a promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||84752||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 8330
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter hU6
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsUse SapI digest to check for unwanted recombination of lentiviral plasmid. Only amplify in RecA- bacteria (eg. Stbl3).
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ sequencing primer hU6-F
- 3′ sequencing primer hGata4-rev (5'-ATTGTGGATGAATACTGCC-3') (Common Sequencing Primers)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Lenti_gRNA-Puro was a gift from Hyongbum Kim (Addgene plasmid # 84752 ; http://n2t.net/addgene:84752 ; RRID:Addgene_84752)
For your References section:In vivo high-throughput profiling of CRISPR-Cpf1 activity. Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, Koh HC, Nam JW, Kim H. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. 10.1038/nmeth.4104 PubMed 27992409