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(Plasmid #161514)


This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 161514 Standard format: Plasmid sent in bacteria as agar stab 1 $85


  • Vector backbone
  • Backbone manufacturer
    Feng Zhang
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
    Prime editor 2-P2A-Blast
  • Alt name
    Cas9(H840A)-M-MLV reversetranscriptase (D200N, T306K, W313F, T330P, L603W)-P2A-BSD
  • Species
  • Insert Size (bp)
  • Promoter EF-1α core promoter
  • Tags / Fusion Proteins
    • SV40 NLS (N terminal on insert)
    • SV40 NLS (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer EFS_forward (5'-GGTCGGCAATTGATCCGGTG-3')
  • 3′ sequencing primer WPRE_reverse (5'-GGTTGCGTCAGCAAACACAG-3')
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-PE2-BSD was a gift from Hyongbum Kim (Addgene plasmid # 161514 ; ; RRID:Addgene_161514)
  • For your References section:

    Predicting the efficiency of prime editing guide RNAs in human cells. Kim HK, Yu G, Park J, Min S, Lee S, Yoon S, Kim HH. Nat Biotechnol. 2020 Sep 21. pii: 10.1038/s41587-020-0677-y. doi: 10.1038/s41587-020-0677-y. 10.1038/s41587-020-0677-y PubMed 32958957