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pLC-Flag-CRBN-P2A-Hygro
(Plasmid #124303)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 124303 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLCE
  • Total vector size (bp) 8895
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CRBN
  • Alt name
    Cereblon
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1347
  • Entrez Gene
    CRBN (a.k.a. MRT2, MRT2A)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag (N terminal on insert)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    CRBN cDNA was PCR amplified from Addgene plasmid 107380.
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLC-Flag-CRBN-P2A-Hygro was a gift from Eva Gottwein (Addgene plasmid # 124303 ; http://n2t.net/addgene:124303 ; RRID:Addgene_124303)
  • For your References section:

    Genome-wide CRISPR screens reveal genetic mediators of cereblon modulator toxicity in primary effusion lymphoma. Patil A, Manzano M, Gottwein E. Blood Adv. 2019 Jul 23;3(14):2105-2117. doi: 10.1182/bloodadvances.2019031732. 10.1182/bloodadvances.2019031732 PubMed 31300418