Purpose(Empty Backbone) Lentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||127749||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size (bp) 10218
Vector typeMammalian Expression, Lentiviral, CRISPR
- Promoter human U6
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsDH10B is also OK.
Copy numberHigh Copy
- Cloning method Restriction Enzyme
For cloning guides, digest the plasmid with Esp3i for the backbone.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:LentiUniversal-Puro was a gift from Giulio Draetta (Addgene plasmid # 127749 ; http://n2t.net/addgene:127749 ; RRID:Addgene_127749)
For your References section:Pooled library screening with multiplexed Cpf1 library. Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G. Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x. 10.1038/s41467-019-10963-x PubMed 31316073