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Pooled library screening with multiplexed Cpf1 library.
Liu J, Srinivasan S, Li CY, Ho IL, Rose J, Shaheen M, Wang G, Yao W, Deem A, Bristow C, Hart T, Draetta G
Nat Commun. 2019 Jul 17;10(1):3144. doi: 10.1038/s41467-019-10963-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
127749LentiUniversal-PuroLentiviral plasmid for expressing any U6 driven transcripts (sgRNAs, crRNAs, shRNAs and etc...)
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127750LentiAsCpf1-3xMYC-blastLentiviral plasmid for expressing human codon-optimized AsCpf1 containing 3x N-terminal MYC-NLS signal
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130630Mini-human AsCpf1-based human genome-wide knockout libraryExpresses crRNAs for genome-wide knockout pooled library screening
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