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Berger Lab Human Paralog Knockout Library (pgPEN)
(Pooled Library #171172)

  • Purpose

    The human pgPEN lentiviral CRISPR library induces knockouts of paralogous genes. Plasmids in the library express a pair of two guide RNAs (pgRNAs). Each construct knocks out an individual gene or pair of paralogs.

  • Vector Backbone

    lentiGuide-Puro - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 171172 Human Paralog Knockout Library (pgPEN) 1 $ 540 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with plentiCas9-Blast (Addgene #52962), Lenti-iCas9-neo (Addgene #85400), or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    1,030 duplicate paralog gene pairs
  • gRNAs

    • 8 pgRNAs for single gene knockout
    • 16 pgRNAs for combined knockouts
  • Controls
    500 non-targeting paired gRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human Paralog Knockout Library (pgPEN) was a gift from Alice Berger (Addgene #171172)
  • For your References section:

    Discovery of synthetic lethal and tumor suppressor paralog pairs in the human genome.. Parrish PCR, Thomas JD, Gabel AM, Kamlapurkar S, Bradley RK, Berger AH. Cell Rep. 2021 Aug 31;36(9):109597. doi: 10.1016/j.celrep.2021.109597. PubMed 34469736