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CRISPR header icon CRISPR/Cas Plasmids: Pooled Libraries


CRISPR/Cas9 is a useful tool for genetic screening experiments, due to the relative ease of designing gRNAs and the ability of Cas9 to modify virtually any genetic locus. CRISPR pooled libraries consist of thousands of plasmids, each containing a gRNA toward a different target gene. In a CRISPR screening experiment, target cells are treated with the pooled library to create a population of “mutant” cells, which are then “screened” for a phenotype of interest. Screening experiments using a pooled CRISPR library are far more complex than using CRISPR to modify a single genomic locus.

Before ordering, please review the steps for using pooled CRISPR libraries and keep the following in mind:

Once you have determined that CRISPR-based screening is appropriate for your experiment, the next step is to pick a library. The pooled CRISPR libraries in Addgene’s collection differ based on some important criteria, and it is important to know which library best suits your particular experiment. Some important variables include:

All of our current pooled CRISPR libraries can be found in the following table, which also includes information about the type of genetic modification and target species. Additional information about each library can be found on the individual library page.

Find Pooled Libraries of Interest

Click on different properties to create a custom filtered list of the CRISPR pooled libraries in Addgene's collection.

Library Type

Species

Library Size

Viral Prep Available

Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs
Activity-optimized genome-wide library 1000000067 Knockout Human Sabatini and Lander 3rd 10 178,896
Broad GPP genome-wide Brunello 73179 (1 plasmid)
73178 (2 plasmid) 
Knockout Human Doench and Root 3rd 4 76,441
Broad GPP genome-wide Brie 73632 (1 plasmid)
73633 (2 plasmid) 
Knockout Mouse Doench and Root 3rd 4 78,637
Broad GPP kinome Brunello 75314, 75315
(1 plasmid)
75312, 75313
(2 plasmid)
Knockout Human Doench and Root 3rd 4 3,052
Broad GPP kinome Brie 75317 (1 plasmid)
75316 (2 plasmid)
Knockout Mouse Doench and Root 3rd 4 2,852
CRiNCL - Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies
CRISPRa Discontinued Activation Human Weissman 3rd 10 198,810
CRISPRa-v2 83978
1000000091
Activation Human Weissman 3rd 5
10
104,540
209,080
CRISPRa-v2 83996
1000000093
Activation Mouse Weissman 3rd 5
10
107,105
214,210
CRISPRi Discontinued Inhibition Human Weissman 3rd 10 206,421
CRISPRi-v2 83969
1000000090
Inhibition Human Weissman 3rd 5
10
104,535
209,070
CRISPRi-v2 83987
1000000092
Inhibition Mouse Weissman 3rd 5
10
107,415
214,830
Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini and Lander 3rd 10 Varies
hCRISPRa-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies
hCRISPRi-v2 subpooled libraries 83971 — 83977 Inhibition Human Weissman 3rd 5 Varies
mCRISPRa-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies
mCRISPRi-v2 subpooled libraries 83989 — 83995 Inhibition Mouse Weissman 3rd 5 Varies
Human GeCKO v2 1000000048
(1 plasmid)
1000000049
(2 plasmid)
Knockout Human Zhang 3rd 6 123,411
Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406
Human improved genome-wide library v1 67989 Knockout Human Yusa 3rd 5 90,709
Mouse GeCKO v2 1000000052
(1 plasmid)
1000000053
(2 plasmid)
Knockout Mouse Zhang 3rd 6 130,209
Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897
Mouse improved genome-wide library v2 67988 Knockout Mouse Yusa 3rd 5 90,230
Oxford Fly 64750 Knockout D. melanogaster Liu N/A 3 40,279
Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A
SAM v1 - 3 plasmid system 1000000057 (Zeocin)
1000000074 (Puromycin)
Activation Human Zhang 3rd 3 70,290
SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716
SAM v2 - 2 plasmid system 1000000078 (Blasticidin) Activation Human Zhang 3rd 3 70,290
Toronto KnockOut 1000000069 Knockout Human Moffat 3rd 12 176,500
Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A 10 8,158

Currently, no available libraries.

Browse all libraries

Name ID Library Type Species PI Lentiviral Generation gRNAs per gene Total gRNAs
Activity-optimized genome-wide library 1000000067 Knockout Human Sabatini and Lander 3rd 10 178,896
Broad GPP genome-wide Brunello 73179 (1 plasmid)
73178 (2 plasmid) 
Knockout Human Doench and Root 3rd 4 76,441
Broad GPP genome-wide Brie 73632 (1 plasmid)
73633 (2 plasmid) 
Knockout Mouse Doench and Root 3rd 4 78,637
Broad GPP kinome Brunello 75314, 75315
(1 plasmid)
75312, 75313
(2 plasmid)
Knockout Human Doench and Root 3rd 4 3,052
Broad GPP kinome Brie 75317 (1 plasmid)
75316 (2 plasmid)
Knockout Mouse Doench and Root 3rd 4 2,852
CRiNCL - Human CRISPRi Non-coding Libraries 86538 — 86550 Inhibition Human Weissman 3rd 10 Varies
CRISPRa Discontinued Activation Human Weissman 3rd 10 198,810
CRISPRa-v2 83978
1000000091
Activation Human Weissman 3rd 5
10
104,540
209,080
CRISPRa-v2 83996
1000000093
Activation Mouse Weissman 3rd 5
10
107,105
214,210
CRISPRi Discontinued Inhibition Human Weissman 3rd 10 206,421
CRISPRi-v2 83969
1000000090
Inhibition Human Weissman 3rd 5
10
104,535
209,070
CRISPRi-v2 83987
1000000092
Inhibition Mouse Weissman 3rd 5
10
107,415
214,830
Enriched subpools (kinase, nuclear, ribosomal, cell cycle) 51043 — 51048 Knockout Human Sabatini and Lander 3rd 10 Varies
hCRISPRa-v2 subpooled libraries 83980 — 83986 Activation Human Weissman 3rd 5 Varies
hCRISPRi-v2 subpooled libraries 83971 — 83977 Inhibition Human Weissman 3rd 5 Varies
mCRISPRa-v2 subpooled libraries 83998 — 84004 Activation Mouse Weissman 3rd 5 Varies
mCRISPRi-v2 subpooled libraries 83989 — 83995 Inhibition Mouse Weissman 3rd 5 Varies
Human GeCKO v2 1000000048
(1 plasmid)
1000000049
(2 plasmid)
Knockout Human Zhang 3rd 6 123,411
Human genome-wide library v1 69763 Knockout Human Wu 3rd 4 77,406
Human improved genome-wide library v1 67989 Knockout Human Yusa 3rd 5 90,709
Mouse GeCKO v2 1000000052
(1 plasmid)
1000000053
(2 plasmid)
Knockout Mouse Zhang 3rd 6 130,209
Mouse genome-wide library v1 Discontinued Knockout Mouse Yusa 3rd 5 87,897
Mouse improved genome-wide library v2 67988 Knockout Mouse Yusa 3rd 5 90,230
Oxford Fly 64750 Knockout D. melanogaster Liu N/A 3 40,279
Perturb-seq Guide Barcodes (GBC) 85968 Barcode Human Weissman 3rd N/A N/A
SAM v1 - 3 plasmid system 1000000057 (Zeocin)
1000000074 (Puromycin)
Activation Human Zhang 3rd 3 70,290
SAM v1 - 3 plasmid system 1000000075 (Puromycin) Activation Mouse Zhang 3rd 3 69,716
SAM v2 - 2 plasmid system 1000000078 (Blasticidin) Activation Human Zhang 3rd 3 70,290
Toronto KnockOut 1000000069 Knockout Human Moffat 3rd 12 176,500
Toxoplasma Knockout 80636 Knockout T. gondii Lourido N/A 10 8,158