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Mouse CRISPR/Cas9-assisted Removal of Mitochondrial DNA (CARM) Library
(Pooled Library #82480)

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  • Purpose

    This library is intended to be used as a technical tool for removal of mitochondrial DNA from mouse genomic DNA preparations.

    This genome engineering process is called “CRISPR/Cas9-assisted removal of mitochondrial DNA (CARM)”.

  • Vector Backbone

    pUC57kan-T7 - this backbone does not contain Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 82480 Mouse gRNA pooled sub-library targeting mitochondrial DNA (pUC57kan-T7-mt-gRNA)† 1 $540 Add to Cart
This material is available to academics and nonprofits only.

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately.

Library Details

  • Species
    Mouse
  • Genes targeted
    gRNAs target every ∼40bp over mitochondrial genome
  • gRNAs
    395
  • Controls
    N/A
  • Lentiviral Generation
    N/A

Library Shipping

This library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

You will receive:

  • Volume
    ∼10µL
  • Concentration
    10ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    pUC57kan-T7-mt-gRNA Library was a gift from Wei Xie (Addgene # 82480).

  • For your References section:

    The landscape of accessible chromatin in mammalian preimplantation embryos. Wu J, Huang B, Chen H, Yin Q, Liu Y, Xiang Y, Zhang B, Liu B, Wang Q, Xia W, Li W, Li Y, Ma J, Peng X, Zheng H, Ming J, Zhang W, Zhang J, Tian G, Xu F, Chang Z, Na J, Yang X, Xie W. Nature. 2016 Jun 30;534(7609):652-7. PubMed 27309802