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Purpose
CRISPR KO library targeting a comprehensive set of mouse genes with annotated function as chromatin regulators.
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Vector Backbone
pXPR_024 (GB, 12 KB) - does not express Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |||
|---|---|---|---|---|---|---|---|
| Pooled Library | 200011 | Mouse Chromatin Regulator Library† | 1 | $ 380 | Add to Cart | ||
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with plasmids expressing Cas9 such as lentiCas9-Blast (#52962) or used with cell lines already expressing Cas9.
Library Details
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SpeciesMouse
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Genes targeted936
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gRNAs per gene6
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gRNAs~5,616
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼25 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit Terms
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Mouse Chromatin Regulator Library was a gift from Gabriel Griffin, Robert Manguso, Bradley Bernstein (Addgene #200011) -
For your References section:
Epigenetic silencing by SETDB1 suppresses tumour intrinsic immunogenicity. Griffin GK, Wu J, Iracheta-Vellve A, Patti JC, Hsu J, Davis T, Dele-Oni D, Du PP, Halawi AG, Ishizuka JJ, Kim SY, Klaeger S, Knudsen NH, Miller BC, Nguyen TH, Olander KE, Papanastasiou M, Rachimi S, Robitschek EJ, Schneider EM, Yeary MD, Zimmer MD, Jaffe JD, Carr SA, Doench JG, Haining WN, Yates KB, Manguso RT, Bernstein BE. Nature. 2021 Jul;595(7866):309-314. doi: 10.1038/s41586-021-03520-4. PubMed 33953401