Mouse Kinome CRISPR Knockout Library (Brie)
(Pooled Libraries #75316, #75317)
- PurposeMouse kinome CRISPR knockout pooled libraries. Each library contains 2,852 unique sgRNAs targeting 713 mouse kinase genes for 4 guides per target.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Mouse Kinome CRISPR pooled library (Brie) was a gift from John Doench & David Root (Addgene # )
For your References section:Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 . Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. 10.1038/nbt.3437 PubMed 26780180