Mouse Kinome CRISPR Knockout Library (Brie)
(Pooled Libraries #75316, #75317)

Library Details

• Species : Mouse
• Genes targeted : 713
• gRNAs : 2,852
• Controls : 100
• Lentiviral Generation : 3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume : ∼10 µL
• Concentration : 10 ng/µL

Resource Information

• Protocols :
  • Library Amplification Protocol (88.2 KB)
  • Sequencing Protocol (158.2 KB)
• Depositor Data :
  • Mouse Kinome Library Target Genes(307 KB)
• Terms and Licenses :
  • UBMTA

Addgene Notes

The amplification protocol provided, which recommends using 400 ng of DNA per transformation, was written in reference to the whole genome-wide libraries. These kinome-specific sub-pools contain fewer plasmids and thus require less DNA to achieve complete representation. Therefore, using the 100 ng of DNA as provided for transformation is sufficient.

NOTE: Lentiviral vectors can recombine during library amplification, resulting in an ~1 kb band containing the origin and ampicillin resistance cassette. This recombination product should not adversely affect library function as it does not contain lentiviral packaging sequences or the PuroR gene and will not be selected during screening. If this 1 kb band is observed after amplification, we recommend starting with a larger prep (gigaprep) from the original sample or gel purifying the amplified library to remove the recombinant band and performing another transformation with the purified sample.