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Mouse Kinome CRISPR Knockout Library (Brie)
(Pooled Libraries #75316, #75317)

  • Purpose
    Mouse kinome CRISPR knockout pooled libraries. Each library contains 2,852 unique sgRNAs targeting 713 mouse kinase genes for 4 guides per target.
  • Vector Backbone
    Available in either a 1 vector (lentiCRISPRv2 backbone) or 2 vector (lentiGuide-Puro backbone) system.

Ordering

1 vector system (lentiCRISPRv2)
Item Catalog # Description Quantity Price (USD)
Pooled Library 75317 gRNAs 1-4 in lentiCRISPRv2 1 $350 Add to Cart
Available to Academic and Nonprofits Only

2 vector system (lentiGuide-Puro)
Item Catalog # Description Quantity Price (USD)
Pooled Library 75316 gRNAs 1-4 in lentiGuide-Puro 1 $350 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with lentiCas9-Blast (Addgene #52962) or otherwise with cell lines already expressing Cas9.

Library Details

  • Species
    Mouse
  • Genes targeted
    713
  • gRNAs
    2,852
  • Controls
    100
  • Lentiviral Generation
    3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼10µL
  • Concentration
    10ng/µL

Addgene Notes

The amplification protocol provided, which recommends using 400ng of DNA per transformation, was written in reference to the whole genome-wide libraries. These kinome-specific sub-pools contain fewer plasmids and thus require less DNA to achieve complete representation. Therefore, using the 100ng of DNA as provided for transformation is sufficient.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Mouse Kinome CRISPR pooled library (Brie) was a gift from John Doench & David Root (Addgene # )
  • For your References section:

    Optimized sgRNA design to maximize activity and minimize off-target effects of CRISPR-Cas9 . Doench JG, Fusi N, Sullender M, Hegde M, Vaimberg EW, Donovan KF, Smith I, Tothova Z, Wilen C, Orchard R, Virgin HW, Listgarten J, Root DE. Nat Biotechnol. 2016 Jan 18. doi: 10.1038/nbt.3437. 10.1038/nbt.3437 PubMed 26780180