Yeo Lab RNA-Binding Protein Pooled CRISPR Knockout Library
(Pooled Library #141438)
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Purpose
This pooled CRISPR knockout library targets RNA-binding proteins with 10 guide RNAs per target.
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Vector Backbone
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Depositing Labs
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Pooled Library | 141438 | RBP CRISPR Knockout Library | 1 | $540 | Add to Cart |
Library Details
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SpeciesHuman
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Genes targeted1,078
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gRNAs12,472
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Controls
Non-targeting controls: 1,058
Essential genes: 628
Fluorescent controls: 12 -
Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Academic/Nonprofit Terms-
Protocols
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Depositor Data
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Articles Citing this Pooled Library
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Terms and Licenses
Academic/Nonprofit TermsIndustry Terms- Not Available to Industry
Trademarks- Zeocin® is an InvivoGen trademark.
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Depositor Comments
The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
RNA-Binding Protein Pooled CRISPR Knockout Library was a gift from Eugene Yeo (Addgene #141438) -
For your References section:
Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors. Wheeler EC, Vu AQ, Einstein JM, DiSalvo M, Ahmed N, Van Nostrand EL, Shishkin AA, Jin W, Allbritton NL, Yeo GW. Nat Methods. 2020 May 11. pii: 10.1038/s41592-020-0826-8. doi: 10.1038/s41592-020-0826-8. PubMed 32393832