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Junjie Chen Lab DNA Damage Response MKOv4 Library
(Pooled Library #140219)

  • Purpose

    This lentiviral CRISPR knockout library targets human genes with known or suspected roles in DNA damage response and DNA repair.

  • Vector Backbone

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 140219 DDR_MKOv4 library 1 $350 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted

    • 365 DDR genes
    • 45 core-essential genes
    • 50 non-essential genes
  • gRNAs
    4,530
  • Controls

    • 443 positive control sgRNAs
    • 500 negative control sgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DDR_MKOv4 library was a gift from Junjie Chen (Addgene #140219)
  • For your References section:

    CRISPR/CAS9-based DNA damage response screens reveal gene-drug interactions. Su D, Feng X, Colic M, Wang Y, Zhang C, Wang C, Tang M, Hart T, Chen J. DNA Repair (Amst). 2020 Jan 16;87:102803. doi: 10.1016/j.dnarep.2020.102803. PubMed 31991288