Garnett Lab MinLibCas9 Library
(Pooled Library #164896)
This human genome-wide CRISPR knockout library is designed with two single guide RNAs (sgRNA) per gene.
The minimal library has been optimized through empirical assessment using comprehensive sgRNA efficiency metrics.
pKLV2-U6gRNA(BbsI)-ccdB-PGKpuro2ABFP-W - does not express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||164896||MinLibCas9 Library†||1||$500||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately.
Controls200 non-targeting sgRNAs
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
The depositing lab recommends calculating total read counts per gRNA using the count_spacers script from the Feng Zhang Lab.
Read distribution per gRNA can be determined using the ineq package in R, calculating both the Lorenz-curve and Gini-coefficient.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Garnett Lab MinLibCas9 Library was a gift from Mathew Garnett (Addgene #164896)
For your References section:Minimal genome-wide human CRISPR-Cas9 library. Goncalves E, Thomas M, Behan FM, Picco G, Pacini C, Allen F, Vinceti A, Sharma M, Jackson DA, Price S, Beaver CM, Dovey O, Parry-Smith D, Iorio F, Parts L, Yusa K, Garnett MJ Genome Biol. 2021 Jan 21;22(1):40. doi: 10.1186/s13059-021-02268-4. PubMed 33478580