Human Two Plasmid Activity-Optimized CRISPR Knockout Library
(Pooled Library #1000000095)
The Sabatini/Lander CRISPR pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.
The library is split into three sub-libraries, each containing 10 sgRNAs per gene. Two libraries have ∼90,000 gRNAs each (hL1nC9 & hL2nC9). One library (hL3nC9) has ∼5000 gRNAs.
- Vector Backbone
pLenti-sgRNA - this backbone does not contain Cas9
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Human Two Plasmid Activity-Optimized CRISPR Knockout Library was a gift from David Sabatini and Eric Lander (Addgene # 1000000095)
For your References section:A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors. Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD. Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741. PubMed 27992415