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Human Two Plasmid Activity-Optimized CRISPR Knockout Library
(Pooled Library #1000000095)

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  • Purpose

    The Sabatini/Lander CRISPR pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.

    The library is split into three sub-libraries, each containing 10 sgRNAs per gene. Two libraries have ∼90,000 gRNAs each (hL1nC9 & hL2nC9). One library (hL3nC9) has ∼5000 gRNAs.

  • Vector Backbone

    pLenti-sgRNA - this backbone does not contain Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000095 Three human gRNA pooled sub-libraries in pLenti-sgRNA† 1 $400 Add to Cart
This material is available to academics and nonprofits only.

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pLenti-Cas9-GFP (Addgene #86145) or otherwise with cell lines already expressing Cas9.

Library Details

  • Species
    Human
  • Genes targeted
    18,543
  • gRNAs
    187,536
  • Controls
    1504
  • Lentiviral Generation
    3rd

Library Shipping

This library will be delivered as three pooled DNA sub-libraries in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

Note that each subpool must be transformed separately.

For each sub-library tube, you will receive:

  • Volume
    ∼20µL
  • Concentration
    30ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Human Two Plasmid Activity-Optimized CRISPR Knockout Library was a gift from David Sabatini and Eric Lander (Addgene # 1000000095)

  • For your References section:

    A genome-wide CRISPR screen identifies a restricted set of HIV host dependency factors. Park RJ, Wang T, Koundakjian D, Hultquist JF, Lamothe-Molina P, Monel B, Schumann K, Yu H, Krupzcak KM, Garcia-Beltran W, Piechocka-Trocha A, Krogan NJ, Marson A, Sabatini DM, Lander ES, Hacohen N, Walker BD. Nat Genet. 2016 Dec 19. doi: 10.1038/ng.3741. PubMed 27992415