Human Activity-Optimized CRISPR Knockout Library (3 sub-libraries in lentiCRISPRv1)
(Pooled Library #1000000100)
The Sabatini/Lander CRISPR pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.
The library is split into three sub-libraries. Two tubes each contain 90,000 gRNAs each or 5 sgRNAs per gene. The third tube contains 5,000 gRNAs.
- Vector Backbone
lentiCRISPR v1- Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages users to use the updated lentiCRISPR v2, which can be requested separately.
- Depositing Labs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000100||Three gRNA pooled sub-libraries in lentiCRISPRv1||1||$400||Add to Cart|
Controls1000 non-targeting, 500 intergenic
This library will be delivered as three pooled DNA sub-libraries in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.
Note that each subpool must be transformed separately.
You will receive two sub-libraries with 90,000 gRNAs each at
You will additionally receive one sub-library (hL3C9) with 5,000 gRNAs each at
Terms and Licenses
The depositing lab notes that the DNA preps should be performed separately and then mixed together during virus production at ratios consistent with the pool size.
When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71049).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Activity-Optimized Human CRISPR Pooled Library was a gift from David Sabatini and Eric Lander (Addgene # 1000000100)
For your References section:Identification and characterization of essential genes in the human genome. Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES, Sabatini DM. Science. 2015 Oct 15. pii: aac7041. PubMed 26472758