Pooled Libraries
Human Activity-Optimized CRISPR Knockout Library (2 sub-libraries in lentiCRISPRv1)

Human Activity-Optimized CRISPR Knockout Library (2 sub-libraries in lentiCRISPRv1)
(Pooled Library #1000000067)

Purpose

Note: This library has been replaced by an updated version (see Depositor Comments below).

The Sabatini/Lander CRISPR pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.

The library is split into two sub-libraries, each containing 5 sgRNAs per gene.
Vector Backbone

lentiCRISPR v1- Note: This plasmid has been discontinued, as an updated version is available. For confirmation of screen hits, Addgene encourages users to use the updated lentiCRISPR v2, which can be requested separately.

Depositing Labs

David Sabatini, Eric Lander
Publication

Wang et al Science. 2015 Oct 15. pii: aac7041. (How to cite )

Ordering

Item	Catalog #	Description	Quantity	Price (USD)
Pooled Library	1000000067	Two gRNA pooled sub-libraries in lentiCRISPRv1	1	Discontinued

Available to Academic and Nonprofits Only

Library Details

Species
Human
Genes targeted
18,166
gRNAs
178,896
Controls
1000
Lentiviral Generation
3rd

Library Shipping

This library will be delivered as two pooled DNA half-libraries in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaws.

Note that each subpool must be transformed separately.

For each half-library tube, you will receive:

Volume
∼15 µL
Concentration
10 ng/µL

Resource Information

Protocols
Depositor Data
- List of targeted genes and sgRNA sequences (10.0 MB)
- Sequencing data (sgRNA read counts for each subpool) (2.7 MB)
Terms and Licenses
- UBMTA

Depositor Comments

Note: This item has been discontinued. The Sabatini and Lander labs have released an updated version of the library with an additional tube of gRNAs here.

Screening process for Sabatini CRISPR knockout library — Diagram of Library Screening Process

When performing sgRNA validation in a Cas9-expressing cell line, the depositing laboratory recommends using plasmid pLenti-sgRNA (Addgene #71409).

Amplified grna population graph — sgRNA diversity after initial amplification

initial grna population graph — sgRNA diversity after infection of a target cell population

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

Example for your Materials & Methods section:
Activity-Optimized Human CRISPR Pooled Library was a gift from David Sabatini and Eric Lander (Addgene # 1000000067)
For your References section:
Identification and characterization of essential genes in the human genome. Wang T, Birsoy K, Hughes NW, Krupczak KM, Post Y, Wei JJ, Lander ES, Sabatini DM. Science. 2015 Oct 15. pii: aac7041. PubMed 26472758

Human Activity-Optimized CRISPR Knockout Library (2 sub-libraries in lentiCRISPRv1)(Pooled Library #1000000067)