Pooled Libraries
Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools)

Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools)
(Pooled Libraries #51043, #51044, #51045, #51046, #51047, #51048)

Purpose
Human CRISPR knockout pooled libraries that have been separated into 6 distinct, enriched sub-pools.
Note that while each sub-pool is enriched for their respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
Vector Backbone
pLX-sgRNA- this backbone does not contain Cas9

Depositing Labs
David Sabatini, Eric Lander
Publication
Wang et al Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12.(How to cite)

Ordering

Item	Catalog #	Description	Quantity
Pooled Library	51043	Unknown function gRNA sub-pool library ^†	1	$400	Add to Cart
Pooled Library	51044	Kinase gRNA sub-pool library ^†	1	$400	Add to Cart
Pooled Library	51045	Ribosomal protein gRNA sub-pool library ^†	1	$400	Add to Cart
Pooled Library	51046	Cell cycle proteins gRNA sub-pool library ^†	1	$400	Add to Cart
Pooled Library	51047	Nuclear proteins gRNA sub-pool library ^†	1	$400	Add to Cart
Pooled Library	51048	Control targets (most diverse) gRNA sub-pool library ^†	1	$350	Add to Cart

Available to Academic and Nonprofits Only

^† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pCW-Cas9 (Addgene#50661) or otherwise with cell lines already expressing Cas9.

Library Details

Species
Human
Genes targeted
Varies
gRNAs
Varies
Controls
See above Pooled Library #51048 enriched control sub-pool
Lentiviral Generation
3rd

Library Shipping

Each library is delivered in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

Volume
∼20µL
Concentration
50ng/µL

Resource Information

Protocols
- Sub-pool Protocol(166 KB)
Depositor Data
- List of genes and sgRNA sequences(9.9 MB)
Terms and Licenses
- UBMTA

Depositor Comments

IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.

There are two lentiviral vectors associated with this paper, one expressing Cas9 ( pCW-Cas9 ) and the sgRNA backbone ( pLX-sgRNA ).

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

Example for your Materials & Methods section:
Human CRISPR enriched pooled library was a gift from David Sabatini & Eric Lander (Addgene # )
For your References section:
Genetic screens in human cells using the CRISPR-Cas9 system . Wang T, Wei JJ, Sabatini DM, Lander ES. Science. 2014 Jan 3;343(6166):80-4. doi: 10.1126/science.1246981. Epub 2013 Dec 12. 10.1126/science.1246981PubMed 24336569

Human CRISPR Knockout Pooled Libraries (Enriched Sub-pools) (Pooled Libraries #51043, #51044, #51045, #51046, #51047, #51048)