Purpose(Empty Backbone) There is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBI
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71409||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerFeng Zhang
- Backbone size (bp) 9100
Modifications to backboneremoved Cas9 from Feng Zhang's pLentiCRISPR plasmid
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Growth Strain(s)NEB Stable
- Cloning method Restriction Enzyme
Articles Citing this Plasmid
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
It is a lentiviral plasmid so it may be recommended to be propagated at 30oc but we typically grow at 37oC.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pLenti-sgRNA was a gift from Eric Lander & David Sabatini (Addgene plasmid # 71409 ; http://n2t.net/addgene:71409 ; RRID:Addgene_71409)