Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library
(Pooled Library #1000000096)
The Sabatini/Lander CRISPR mouse pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.
The library is split into two sub-libraries. Each library contains ~90,000 gRNAs.
- Vector Backbone
pLenti-sgRNA - this backbone does not contain Cas9
- Depositing Labs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000096||Two mouse gRNA pooled sub-libraries in pLenti-sgRNA†||1||$400||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pLenti-Cas9-GFP (Addgene #86145) or otherwise with cell lines already expressing Cas9.
This library will be delivered as two pooled DNA sub-libraries in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.
Note that each subpool must be transformed separately.
For each half-library tube, you will receive:
Terms and Licenses
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library was a gift from David Sabatini and Eric Lander (Addgene # 1000000096)
For your References section:Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM. Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. PubMed 28162770