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Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library
(Pooled Library #1000000096)

  • Purpose

    The Sabatini/Lander CRISPR mouse pooled library is optimized for cleavage activity, in order to maximize the likelihood of gene knockout.

    The library is split into two sub-libraries. Each library contains ~90,000 gRNAs.

  • Vector Backbone

    pLenti-sgRNA - this backbone does not contain Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000096 Two mouse gRNA pooled sub-libraries in pLenti-sgRNA 1 $400 Add to Cart
This material is available to academics and nonprofits only.
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pLenti-Cas9-GFP (Addgene #86145) or otherwise with cell lines already expressing Cas9.

Library Details

  • Species
    Mouse
  • Genes targeted
    18,986
  • gRNAs
    188,509
  • Controls
    199
  • Lentiviral Generation
    3rd

Library Shipping

This library will be delivered as two pooled DNA sub-libraries in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

Note that each subpool must be transformed separately.

For each half-library tube, you will receive:

  • Volume
    ∼20µL
  • Concentration
    30ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Mouse Two Plasmid Activity-Optimized CRISPR Knockout Library was a gift from David Sabatini and Eric Lander (Addgene # 1000000096)
  • For your References section:

    Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras. Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM. Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. PubMed 28162770