Gene Essentiality Profiling Reveals Gene Networks and Synthetic Lethal Interactions with Oncogenic Ras.
Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM
Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. PubMed Article
Wang T, Yu H, Hughes NW, Liu B, Kendirli A, Klein K, Chen WW, Lander ES, Sabatini DM
Cell. 2017 Feb 1. pii: S0092-8674(17)30061-2. doi: 10.1016/j.cell.2017.01.013. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 86122 | pMXs-HA-C1orf27 | Retroviral vector expressing HA-C1orf27-IRES-Bsd |
| 86124 | pMXs-RAP2A-GFP | Retroviral vector expressing RAP2A-GFP-IRES-Bsd |
| 86125 | pMXs-NDUFAF5-HA | Retroviral vector expressing NDUFAF5-HA-IRES-Bsd |
| 86127 | LentiCRISPR-sgPREX1 | Lentiviral vector expressing Cas9 and an sgRNA targeting PREX1 |
| 86128 | LentiCRISPR-sgSHOC2-1 | Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2 |
| 86129 | LentiCRISPR-sgSHOC2-2 | Lentiviral vector expressing Cas9 and an sgRNA targeting SHOC2 |
| 86130 | LentiCRISPR-sgC1orf27-1 | Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27 |
| 86131 | LentiCRISPR-sgC1orf27-2 | Lentiviral vector expressing Cas9 and an sgRNA targeting C1orf27 |
| 86132 | LentiCRISPR-sgUBA5 | Lentiviral vector expressing Cas9 and an sgRNA targeting UBA5 |
| 86133 | LentiCRISPR-sgUFM1 | Lentiviral vector expressing Cas9 and an sgRNA targeting UFM1 |
| 86134 | LentiCRISPR-sgUFSP2 | Lentiviral vector expressing Cas9 and an sgRNA targeting UFSP2 |
| 86135 | LentiCRISPR-sgC17orf89-2 | Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89 |
| 86136 | LentiCRISPR-sgC17orf89-3 | Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89 |
| 86137 | LentiCRISPR-sgC17orf89-1 | Lentiviral vector expressing Cas9 and an sgRNA targeting C17orf89 |
| 86138 | pMXs2-RAC1 | Retrolviral vector expressing RAC1-IRES-Bsd-p2A-RFP |
| 86139 | pMXs2-RAC1 G12V | Retroviral vector expressing RAC1(G12V)-IRES-Bsd-p2A-RFP |
| 86141 | pMXs2-MEK1 | Retroviral vector expressing MEK1-IRES-Bsd-p2A-RFP |
| 86143 | pMXs3-TIAM1 | Retroviral vector expressing TIAM1-IRES-RFP |
| 86144 | pMXs3-NRAS G13D | Retroviral vector expressing NRAS(G13D)-IRES-RFP |
| 86145 | pLenti-Cas9-GFP | Lentiviral vector expressing Cas9-GFP |
| 92352 | Sabatini/Lander Lab Ras synthetic lethal candidate focused human sgRNA library in lentiCRISPRv1 | contains 6,661 sgRNAs (comprising 499 intergenic control sgRNAs and 6,162 sgRNAs targeting 132 control and candidate Ras synthetic lethal genes at up to 50 sgRNAs per gene) |