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Toronto KnockOut (TKO) CRISPR Library - Version 3
(Pooled Library #90294)

  • Purpose

    Human CRISPR lentiviral library that has 70,948 guides (4 guides/gene) targeting 18,053 protein-coding genes.

  • Vector Backbone
    lentiCRISPRv2 - this backbone contains Cas9


Item Catalog # Description Quantity Price (USD)
Pooled Library 90294 gRNA knockout pooled library 1 $500 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
  • Genes targeted
  • gRNAs
  • Controls
    142 guides targeting LacZ, EGFP, and luciferase
  • Lentiviral Generation

Library Shipping

This library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
  • Concentration

Resource Information

Depositor Comments

The Toronto KnockOut Library v3 (TKOv3) contains 70,948 gRNAs targeting 18,053 protein coding genes (4 gRNAs/gene) with 142 control gRNAs targeting EGFP, LacZ, and luciferase (71,090 total).

The gRNAs are sequence-optimized based on the criteria described in the associated article, and the number of gRNAs per gene is decreased to 4 based on the finding that there are diminishing benefits to more gRNAs per gene beyond four.

Additionally, this library contains Cas9 in the library plasmids, thus making it a 1 plasmid system, without the need to include a separate Cas9. Thus, TKOv3 offers the benefit of improved accuracy, efficiency, and scalability for CRISPR screens.

Please see for bioRxiv preprint describing this library.

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How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

  • Example for your Materials & Methods section:

    Toronto human knockout pooled library (TKOv3) was a gift from Jason Moffat (Addgene #90294)
  • For your References section:

    Evaluation and Design of Genome-Wide CRISPR/SpCas9 Knockout Screens. Hart T, Tong AHY, Chan K, Van Leeuwen J, Seetharaman A, Aregger M, Chandrashekhar M, Hustedt N, Seth S, Noonan A, Habsid A, Sizova O, Nedyalkova L, Climie R, Tworzyanski L, Lawson K, Sartori MA, Alibeh S, Tieu D, Masud S, Mero P, Weiss A, Brown KR, Usaj M, Billmann M, Rahman M, Constanzo M, Myers CL, Andrews BJ, Boone C, Durocher D, Moffat J. G3 (Bethesda). Aug 7;7(8):2719-2727. doi: 10.1534/g3.117.041277. PubMed 28655737.