Toronto KnockOut (TKO) CRISPR Library - Version 1
(Pooled Library #1000000069)

Library Details

• Species : Human
• Genes targeted : 17,661
• gRNAs : 176,500
  • Base 90k library: 91,320
  • Supplemental library: 85,180
• Controls : Targets include LacZ, EGFP, luciferase, and random loci on chromosome 10
• Lentiviral Generation : 3rd

Library Shipping

This library will be delivered as two pooled DNA half-libraries in microcentrifuge tubes on blue ice. The tubes' contents will not necessarily be frozen. For best results, minimize freeze-thaws.

For each half-library, you will receive:

• Volume : ∼20µL
• Concentration : 50ng/µL

Resource Information

• Depositor Data :
  

  Please visit the Moffat Toronto KnockOut (TKO) library website for the most up-to-date information, including:

  • library sequences and targets
  • read counts
  • RNA-seq data

  The Moffat Lab also provides a gRNA viewer , that displays gRNA sequence information for a specific gene.

  Note that each subpool must be transformed separately.

• Terms and Licenses :
  • UBMTA

Depositor Comments

One sub-library (base “90k library”) was used in Hart et al (2015) as a first-pass and contains 91,320 sequences. The sgRNAs selected for this library had no genomic off-target sites. Control guides target LacZ, EGFP, luciferase, and random loci on chromosome 10. Additionally, 796 guides identified as promisicuous (20+ predicted binding sites) have been included.

The second sub-library (“supplemental library”) was used in Hart et al (2015) as a second-pass and contains 85,180 sequences. sgRNAs included in this library were selected with the parameter that one potential off-target hit was allowed.

Additionally, the Moffat Lab has deposited the Cas9 and gRNA backbone used in their screen.

• gRNA backbone:pLCKO
• FLAG-tagged Cas9:Lenti-Cas9-2A-Blast

These plasmids are NOT included with library purchase and must be ordered separately.

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