Toronto KnockOut (TKO) CRISPR Library
(Pooled Library #1000000069)
Human CRISPR lentiviral library that has 176,500 guides (12 guides/gene) targeting 17,661 protein-coding genes.
The library is split into two sub-libraries, each containing 6 sgRNAs per gene. One sub-library (base “90k library”) contains 91,320 sequences and the second sub-library (“supplemental library”) contains 85,180 sequences.
- Vector BackbonepLCKO- this gRNA backbone does not contain Cas9
- Depositing Labs
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Toronto human knockout pooled library (TKO) was a gift from Jason Moffat (Addgene #1000000069)
For your References section:High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities . Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J. Cell. 2015 Dec 3;163(6):1515-26. 10.1016/j.cell.2015.11.015.PubMed 26627737