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High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.
Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
73310Lenti‐Cas9‐2A‐BlastLentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter
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73311pLCKOLentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
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74179pLCKO_LacZ_sgRNAlentiviral vector expressing sgRNA targeting LacZ
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74180pLCKO_PSMD1_sgRNA_1lentiviral vector expressing sgRNA targeting PSMD1
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74181pLCKO_PSMD1_sgRNA_5lentiviral vector expressing sgRNA targeting PSMD1
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74184pLCKO_PSMB2_sgRNA_1lentiviral vector expressing sgRNA targeting PSMB2
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74185pLCKO_PSMB2_sgRNA_5lentiviral vector expressing sgRNA targeting PSMB2
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74187pLCKO_EIF3D_sgRNA_1lentiviral vector expressing sgRNA targeting EIF3D
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74188pLCKO_EIF3D_sgRNA_4lentiviral vector expressing sgRNA targeting EIF3D
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74190pLCKO_Luciferase_sgRNAlentiviral vector expressing sgRNA targeting Luciferase
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