High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities.
Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J
Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 73310 | Lenti‐Cas9‐2A‐Blast | Lentiviral vector expressing human codon-optimized S. pyogenes FLAG-tagged Cas9 and blasticidin resistance from EFS promoter |
| 73311 | pLCKO | Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection. |
| 74179 | pLCKO_LacZ_sgRNA | lentiviral vector expressing sgRNA targeting LacZ |
| 74180 | pLCKO_PSMD1_sgRNA_1 | lentiviral vector expressing sgRNA targeting PSMD1 |
| 74181 | pLCKO_PSMD1_sgRNA_5 | lentiviral vector expressing sgRNA targeting PSMD1 |
| 74184 | pLCKO_PSMB2_sgRNA_1 | lentiviral vector expressing sgRNA targeting PSMB2 |
| 74185 | pLCKO_PSMB2_sgRNA_5 | lentiviral vector expressing sgRNA targeting PSMB2 |
| 74187 | pLCKO_EIF3D_sgRNA_1 | lentiviral vector expressing sgRNA targeting EIF3D |
| 74188 | pLCKO_EIF3D_sgRNA_4 | lentiviral vector expressing sgRNA targeting EIF3D |
| 74190 | pLCKO_Luciferase_sgRNA | lentiviral vector expressing sgRNA targeting Luciferase |