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Mouse Cherry Brie Pooled Library
(Pooled Library #170511)

  • Purpose

    Lentiviral pooled library for whole-genome CRISPR screening in mouse cells. The library is identical to the Mouse CRISPR Knockout Pooled Library (Brie), Addgene #73633, with an mCherry cassette in place of the Puromycin resistance cassette. Screening requires expression of Cas9 from a separate source.

  • Vector Backbone

    LentiGuide Cherry - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 170511 gRNA pooled library 1 $500 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with LentiCas9-Blast (Addgene #52962) or any other plasmid(s) or cell lines expressing Cas9.

Library Details

  • Species
    Mouse
  • Genes targeted
    19,674
  • gRNAs
    78,637 (~4 gRNA/gene)
  • Controls
    1,000
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Cherry Brie pooled library was a gift from Pavel Tolar (Addgene #170511)
  • For your References section:

    Genome-wide screens identify calcium signaling as a key regulator of IgE+ plasma cell differentiation and survival. Newman R, Tolar P. bioRxiv 2021.03.02.433398. doi: 10.1101/2021.03.02.433398. bioRxiv 2021.03.02.433398