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Li Human UBDUB CRISPR Knockout Library
(Pooled Library #171531)

  • Purpose

    Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP is a pooled gRNA library containing 9,274 individual gRNAs against ~1,500 human ubiquitination and de-ubiquitination complex genes (UBDUB). These were constructed in a lentiviral backbone to allow researchers to conduct comprehensive loss-of-function screens in different contexts.

  • Vector Backbone

    LentiGuide-puromycin-IRES-CFP was prepared by inserting an IRES-CFP cassette into LentiGuide-Puro. This vector does not express Cas9.

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 171531 Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP pooled library 1 $400 Add to Cart
Available to Academic and Nonprofits Only

  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Any SpCas9-expressing plasmid can be used with this library.

Library Details

  • Species
    Human
  • Genes targeted
    1500
  • gRNAs
    9,274 individual gRNAs
  • Controls
    1,000 non-targeting gRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20µL
  • Concentration
    50ng/µL

Depositor Comments

graphic summary of UBDUB CRISPR knockout library
Chunliang Li lab UBDUB CRISPR knockout library

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms and DESeq2 for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human UBDUB CRISPR knockout library was a gift from Chunliang Li (Addgene #171531)
  • For your References section:

    Pooled gRNA library against human ubiquitination and de-ubiquitination complex genes (UBDUB). Chunliang Li. unpublished.