Li Human UBDUB CRISPR Knockout Library
(Pooled Library #171531)

• Purpose:

  Lenti-human-UBDUB-gRNA-puromycin-IRES-CFP is a pooled gRNA library containing 9,274 individual gRNAs against ~1,500 human ubiquitination and de-ubiquitination complex genes (UBDUB). These were constructed in a lentiviral backbone to allow researchers to conduct comprehensive loss-of-function screens in different contexts.

• Vector Backbone:

  LentiGuide-puromycin-IRES-CFP was prepared by inserting an IRES-CFP cassette into LentiGuide-Puro. This vector does not express Cas9.

• Depositing Labs: Chunliang Li
• Publication: Pooled gRNA library against human ubiquitination and de-ubiquitination complex genes (UBDUB).

Library Details

• Species: Human
• Genes targeted: 1500
• gRNAs: 9,274 individual gRNAs
• Controls: 1,000 non-targeting gRNAs
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20µL
• Concentration: 50ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (13.2 KB)
  • Library Construction and Screening Protocol (16.2 KB)
  • Primers and PCR protocol for Illumina sequencing preparation (9.4 KB)
• Depositor Data:
  • List of targeted genes and sgRNA sequences (369.3 KB)
  • Representative Illumina sequencing results (400.7 KB)
• Terms and Licenses:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials

Depositor Comments

Chunliang Li lab UBDUB CRISPR knockout library

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms and DESeq2 for analysis.