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Human Transcription Factor Knockout Library
(Pooled Library #162275)

  • Purpose

    This lentiviral CRISPR knockout library targets 1,639 human transcription factors for comprehensive loss-of-function screens.

  • Vector Backbone

    lentiGuide-Puro - does not express Cas9


Item Catalog # Description Quantity Price (USD)
Pooled Library 162275 Lenti-human-TF-gRNA-puromycin 1 $ 380 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Plasmid(s) such as lentiCas9-Blast (Addgene #52962) or cell lines expressing SpCas9 can be used.

Library Details

  • Species
  • Genes targeted
  • gRNAs
  • Controls
    100 non-targeting gRNAs
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
  • Concentration

Depositor Comments

  • Chunliang Li lab human transcription factor CRISPR knockout library

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms and DESeq2 for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Lenti-human-TF-gRNA-puromycin library was a gift from Chunliang Li (Addgene #162275)
  • For your References section:

    Functional interrogation of HOXA9 regulome in MLLr leukemia via reporter-based CRISPR/Cas9 screen. Zhang H, Zhang Y, Zhou X, Wright S, Hyle J, Zhao L, An J, Zhao X, Shao Y, Xu B, Lee HM, Chen T, Zhou Y, Chen X, Lu R, Li C. Elife. 2020 Oct 1;9. pii: 57858. doi: 10.7554/eLife.57858. PubMed 33001025