Borodina Yeast Transporter Libraries
(Pooled Libraries #153101, 153102, 153103, 153104, 153105, 153106)
These yeast pooled CRISPR libraries target all 361 non-essential and conditionally essential transporter genes in S. cerevisiae. Plasmids contain both an sgRNA and corresponding 165 bp donor fragment, achieving high efficiency disruption of the target genes by introduction of frameshift mutations.
Libraries are divided into five sub-groups targeting different groups of transporters.
- Sctrans-PP-Grp1: ATP dependent transporters
- Sctrans-PP-Grp2: Secondary transporters (SeTS-1 to SeTS-5)
- Sctrans-PP-Grp3: Secondary transporters (SeTS-6 to SeTS-9)
- Sctrans-PP-Grp4: Secondary transporters (SeTS-10 to SeTS-14)
- Sctrans-PP-Grp5: Ion channel, unclassified, and other transporters
- Sctrans-PP targets all 361 genes
pMEL10 - does not express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||153102||Sctrans-PP-Grp1†||1||$300||Add to Cart|
|Pooled Library||153103||Sctrans-PP-Grp2†||1||$300||Add to Cart|
|Pooled Library||153104||Sctrans-PP-Grp3†||1||$300||Add to Cart|
|Pooled Library||153105||Sctrans-PP-Grp4†||1||$300||Add to Cart|
|Pooled Library||153106||Sctrans-PP-Grp5†||1||$300||Add to Cart|
|Pooled Library||153101||Sctrans-PP†||1||$350||Add to Cart|
† A Cas9 plasmid is NOT included with this item. These libraries should be used with a yeast strain constitutively expressing SpCas9.
|Pooled Library||Name||Genes Targeted|
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
Terms and Licenses
The depositing lab's raw NGS data can be downloaded from the European Nucleotide Archive (ENA): Project: PRJEB39414.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Yeast Transporter Library was a gift from Irina Borodina (Addgene #)
For your References section:Transportome-wide engineering of Saccharomyces cerevisiae. Wang G, Moller-Hansen I, Babaei M, D'Ambrosio V, Christensen HB, Darbani B, Jensen MK, Borodina I. Metab Eng. 2021 Jan 16. pii: S1096-7176(21)00015-X. doi: 10.1016/j.ymben.2021.01.007. PubMed 33465478