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Mouse Liver CRISPR Knockout Library
(Pooled Library #192824)

  • Purpose

    This library targets 13,189 of the 13,266 protein-coding genes expressed in the mouse liver at any time point in development, adulthood, and regeneration, as determined by RNAseq (FPKM > 0.3) alongside 6,500 control sgRNAs. This includes an average of 5 sgRNAs per gene, 2,000 non-targeting control sgRNAs and 4,500 control-gene-targeting sgRNAs for a total of 71,878 sgRNAs. It can be used for genome-scale screening in the mouse liver.

  • Vector Backbone

    pLentiCRISPRv2-Stuffer-HepmCherry - does not express Cas9


Item Catalog # Description Quantity Price (USD)
Pooled Library 192824 Mouse Liver CRISPR Knockout Library 1 $ 540 Add to Cart
Available to Academic and Nonprofits Only
  • A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with any *Streptococcus pyogenes* Cas9 (spCas9), such as lentiCas9-Blast (#52962), including inducible spCas9 mice.

Library Details

  • Species
  • Genes targeted
  • gRNAs
  • Controls

    • 6,500 total
    • 2,000 non-targeting
    • 4,500 targeting control genes
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL
How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse Liver CRISPR Knockout library was a gift from Kristin Knouse (Addgene #192824)
  • For your References section:

    Genome-scale CRISPR screening in a single mouse liver. Keys HR, Knouse KA. Cell Genomics. 2022 Nov 15;100217. doi: 10.1016/j.xgen.2022.100217. PubMed xxxx