pLentiCRISPRv2-Stuffer-HepmCherry
(Plasmid
#192825)
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PurposeContains stuffer sequence into which sgRNAs can be cloned and expresses mCherry from hepatocyte-specific promoter
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 192825 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLentiCRISPRv2-Opti
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Backbone manufacturerAddgene (163126)
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Vector typeMammalian Expression, Lentiviral, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemCherry
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SpeciesOther
- Promoter Hepatocyte-specific promoter (HS-CRM8-TTRmin module from Chuah et al. Molecular Therapy 2014)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLentiCRISPRv2-Stuffer-HepmCherry was a gift from Kristin Knouse (Addgene plasmid # 192825 ; http://n2t.net/addgene:192825 ; RRID:Addgene_192825) -
For your References section:
Genome-scale CRISPR screening in a single mouse liver. Keys HR, Knouse KA. Cell Genom. 2022 Dec 14;2(12):100217. doi: 10.1016/j.xgen.2022.100217. Epub 2022 Nov 15. 10.1016/j.xgen.2022.100217 PubMed 36643909
Map uploaded by the depositor.
