Bonifacino Lab Human ubiquitination-related proteins CRISPR KO library
(Pooled Library #174592)
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Purpose
This lentiviral CRISPR knockout library targets ubiquitination-related proteins, including all major E1, E2, E3, and deubiquitinating enzymes.
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Vector Backbone
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 174592 | Human ubiquitination-related KO library | 1 | $ 430 | Add to Cart | |
Available to Academic and Nonprofits Only
Library Details
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SpeciesHuman
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Genes targeted660
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gRNAs11,108
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Controls1,000 non-targetting sgRNAs
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20µL
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Concentration50ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
How to cite this pooled library
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These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Human ubiquitination-related proteins CRISPR KO library was a gift from Juan Bonifacino (Addgene #174592) -
For your References section:
Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3. Jia R, Bonifacino JS. Elife. 2019 Nov 6;8. pii: 50034. doi: 10.7554/eLife.50034. PubMed 31692446