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Bonifacino Lab Human Ubiquitination-Related Proteins CRISPR Knockout Library
(Pooled Library #174592)

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  • Purpose

    This lentiviral CRISPR knockout library targets ubiquitination-related proteins, including all major E1, E2, E3, and deubiquitinating enzymes.

  • Vector Backbone

    lentiCRISPR v2 (Plasmid #52961) - expresses Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 174592 Human Ubiquitination-Related CRISPR KO Library 1 $430 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
    Human
  • Genes targeted
    660
  • gRNAs
    11,108
  • Controls
    1,000 non-targetting sgRNAs
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Human ubiquitination-related proteins CRISPR KO library was a gift from Juan Bonifacino (Addgene #174592 ; http://n2t.net/addgene:174592 ; RRID:Addgene_174592)

  • For your References section:

    Negative regulation of autophagy by UBA6-BIRC6-mediated ubiquitination of LC3. Jia R, Bonifacino JS. Elife. 2019 Nov 6;8. pii: 50034. doi: 10.7554/eLife.50034. PubMed 31692446
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