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Mali Lab Pan-Druggable Cancer Library
(Pooled Library #182133)

  • Purpose

    This library provides an all-in-one CRISPR/Cas system composed of 74 elements: 63 sgRNAs targeting cancer-related genes with FDA-approved drug treatments, 3 sgRNAs targeting key medulloblastoma driver genes, 4 sgRNAs targeting the AAVS1 locus, and 4 non-targeting controls.

  • Vector Backbone

    The LentiCRISPR v2-GFP backbone was prepared by replacing the puromycin-resistance domain from LentiCRISPR v2 with an EGFP reporter sequence.


Item Catalog # Description Quantity Price (USD)
Pooled Library 182133 Pan-Druggable Cancer Library 1 $380 Add to Cart
Available to Academic and Nonprofits Only

Library Details

  • Species
  • Genes targeted
  • gRNAs
  • Controls
    4 sgRNAs targeting the AAVS1 locus, 4 non-targeting controls
  • Lentiviral Generation

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

  • Volume
    ∼20 µL
  • Concentration
    50 ng/µL

Resource Information

Depositor Comments

Schematic of library target selection and list of genes
  • Pan-Druggable Cancer Library design. Left: Library was designed by compiling a list of FDA-Approved cancer drugs, listing the proteins targeted by these drugs, then designing sgRNAs targeting these genes. Right: List of targeted genes, with genes associated as medulloblastoma drivers highlighted in blue. Figure adapted from Hu et al. 2022.

The depositing lab recommends using MAGeCK/MAGeCK-VISPR algorithms (Link opens in a new window) for analysis.

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Pan-Druggable Cancer Library was a gift from Prashant Mali (Addgene #182133)
  • For your References section:

    Integrated genome and tissue engineering enables screening of cancer vulnerabilities in physiologically relevant perfusable ex vivo cultures. Hu M, Lei XY, Larson JD, McAlonis M, Ford K, McDonald D, Mach K, Rusert JM, Wechsler-Reya RJ, Mali P. Biomaterials. 2022 Jan;280:121276. doi: 10.1016/j.biomaterials.2021.121276. PMID 34890975.