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Purpose
This lentiviral murine CRISPR-Cas9 knockout (MusCK) library contains 49,252 guide RNAs targeting 4,922 mouse genes implicated in tumor initiation, progression, and immune modulation and 1000 control gRNAs in each half-library. The library is divided into 2 parts (MusCK Library A and MusCK Library B). The A and B libraries contain in total 10 gRNAs per gene (5 gRNAs in each half-library). Researchers only need an individual library for an experiment.
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Vector Backbone
lentiCRISPR v2 - Expresses Cas9
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 1000000178 | Two tubes, each with a half-library (Library A and B) in backbone lentiCRISPR v2
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1 | $ 750 | Add to Cart | |
| Pooled Library | 174196 | Library A, one tube, contains Liu Lab MusCK Library A | 1 | $ 540 | Add to Cart | |
| Pooled Library | 174197 | Library B, one tube, contains Liu Lab MusCK Library B | 1 | $ 540 | Add to Cart | |
Library Details
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SpeciesMouse
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Genes targeted4,922
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gRNAs24,622 (Library A), 25,630 (Library B)
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Controls2000 control gRNAs (1000 in each half-library)
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Depositor Comments
The depositing lab recommends using MAGeCK algorithms for analysis.
Please visit https://doi.org/10.1101/2020.12.09.418012 for bioRxiv preprint.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Liu Lab MusCK Library was a gift from Xiaole Shirley Liu (Addgene #) -
For your References section:
In vivo CRISPR screens identify the E3 ligase Cop1 as a modulator of macrophage infiltration and cancer immunotherapy target. Wang X, Tokheim C, Gu SS, Wang B, Tang Q, Li Y, Traugh N, Zeng Z, Zhang Y, Li Z, Zhang B, Fu J, Xiao T, Li W, Meyer CA, Chu J, Jiang P, Cejas P, Lim K, Long H, Brown M, Liu XS. Cell. 2021 Oct 14;184(21):5357-5374.e22. doi: 10.1016/j.cell.2021.09.006. PMID: 34582788.