Chen Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library
(Pooled Library #123361)
This lentiviral library is a CRISPR-Cpf1/Cas12a double knockout library. There are 26 genes targeted in the library, chosen from analysis of human metastasis cohorts and a prior mouse metastasis CRISPR screen. It can be used to interrogate the genetic interactions driving metastasis.
pRG01-U6-DR-crRNA-BsmbI(x2)-6T; EFS-Puro-2A-Fluc-WPRE - does not contain Cpf1/Cas12a
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||123361||pRG01-MCAP_MET pooled library†||1||$350||Add to Cart|
† A Cpf1/Cas12a plasmid is NOT included with this item and will have to be ordered separately. Can be used in conjunction with pRC10-EFS-huLbCpf1-2A-Blast-WPRE (Addgene #123359) or any other plasmid(s) or cell lines expressing Cpf1/Cas12a.
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
26 unique genes are targeted, corresponding to 325 unique gene pairs. Each gene has 4 targeting crRNAs. Combined with 52 non-targeting crRNAs, this means there are 1326 NTC-NTC dual-crRNA arrays, 5408 single knockout dual-crRNA arrays (208 per gene), and 5200 double knockout dual-crRNA arrays (16 per gene pair).
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Mouse Metastasis CRISPR-Cpf1(Cas12a) Double Knockout Library was a gift from Sidi Chen (Addgene #123361)
For your References section:In vivo profiling of metastatic double knockouts through CRISPR-Cpf1 screens. Chow RD, Wang G, Ye L, Codina A, Kim HR, Shen L, Dong MB, Errami Y, Chen S. Nat Methods. 2019 May;16(5):405-408. doi: 10.1038/s41592-019-0371-5. PubMed 30962622