Liu Mouse CRISPR Knockout Library in lentiCRISPR v2-Blast
(Pooled Library #1000000173)
This lentiviral genome-wide CRISPR knockout library targets 18,741 mouse genes with 10 guide RNAs per gene. The library is divided into two parts (M1 and M2) with 5 gRNAs per gene in each half-library.
The guide RNA design for this library was optimized to maximize on-target cleavage effects and minimize off-target effects.
lentiCRISPR v2-Blast - expresses Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||1000000173||Liu Mouse CRISPR Knockout Library in lentiCRISPR v2-Blast||1||$500||Add to Cart|
Controls341 control gRNAs
This library will be delivered as two pooled DNA half-libraries in microcentrifuge tubes on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws. For each half-library tube, you will receive:
The depositing lab recommends using MAGeCK algorithms for analysis.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Liu Mouse CRISPR Knockout Library in lentiCRISPR v2-blast was a gift from Xiaole Shirley Liu (Addgene #1000000173)
For your References section:Therapeutically increasing MHC-I expression potentiates immune checkpoint blockade. Gu SS, Zhang W, Wang X, Jiang P, Traugh N, Li Z, Meyer C, Stewig B, Xie Y, Bu X, Manos MP, Font-Tello A, Gjini E, Lako A, Lim K, Conway J, Tewari AK, Zeng Z, Das Sahu A, Tokheim C, Weirather JL, Fu J, Zhang Y, Kroger B, Liang JH, Cejas P, Freeman GJ, Rodig S, Long HW, Gewurz BE, Hodi FS, Brown M, Liu XS. Cancer Discov. 2021 Feb 15. pii: 2159-8290.CD-20-0812. doi: 10.1158/2159-8290.CD-20-0812. 10.1158/2159-8290.CD-20-0812 PubMed 33589424