Superti-Furga Lab Human SLC Knockout Library
(Pooled Library #132552)

• Purpose:

  The CRISPR knockout library targets the human solute carrier family of proteins. A set of additional 20 essential genes is also targeted as a control set.

• Vector Backbone:

  lentiCRISPR v2 - Expresses Cas9

• Depositing Labs: Giulio Superti-Furga
• Publication: Girardi et al Nat Chem Biol. 2020 Apr;16(4):469-478. doi: 10.1038/s41589-020-0483-3. Epub 2020 Mar 9.

Library Details

• Species: Human
• Genes targeted:
  

  390 SLCs
  20 essential genes
  7 pseudogenes

• gRNAs: 2,609
• Controls: 120 non-targeting sgRNAs
• Lentiviral Generation: 3rd

Library Shipping

This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.

• Volume: ∼20µL
• Concentration: 50ng/µL

Resource Information

• Protocols:
  • Library Amplification Protocol (30 KB)
  • Library Sequencing Protocol (90 KB)
• Depositor Data:
  • CRISPR Library NGS Primers (17 KB)
  • List of gRNA Sequences (87 KB)
  • Table of NGS Read Counts (44 KB)
• Terms and Licenses:
  • UBMTA

Depositor Comments

• Superti-Furga Lab Human CRISPR SLC Knockout Library

The library results can be analyzed with several packages, including the MAGeCK algorithms or R/DESeq.

The Research Empowerment on Solute Carriers (ReSOLUTE) plasmid collection contains codon-optimized human SLCs in Gateway-compatible pENTR vectors and may be a useful resource for researchers using this library.