Superti-Furga Lab Human SLC Activation Library
(Pooled Library #132561)
This CRISPR transcriptional activation library targets the human solute carrier family of proteins.
lenti sgRNA(MS2)_zeo backbone - does not express Cas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||132561||gRNA pooled library†||1||$350||Add to Cart|
† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Use in conjunction with lenti dCAS-VP64_Blast (Addgene #61425) and lenti MS2-P65-HSF1_Hygro (Addgene #61426), pPB-R1R2_EF1aVP64dCas9VP64_T2A_MS2p65HSF1-IRESbsdpA (Addgene #113341) or any other plasmid(s) or cell lines expressing both dCas9-VP64 and MS2-p65-HSF1.
Controls120 non-targeting controls
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
The Research Empowerment on Solute Carriers (ReSOLUTE) plasmid collection contains codon-optimized human SLCs in Gateway-compatible pENTR vectors and may be a useful resource for researchers using this library.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:Human SLC Activation Library was a gift from Giulio Superti-Furga (Addgene #132561)