Chong Zhang E. coli Genome-wide Inhibition Library
(Pooled Libraries #113134, #113135, #113136, #113137, #113138, #113146, #113147)
This genome-scale CRISPR inhibition library targets ~4,000 E. coli genes with ~15 gRNAs per gene and includes ~10,000 gRNAs targeting intergenic regions. Additionally, 2,400 gRNAs without any significant hit across the E. coli genome are provided as a negative control. The library is divided into 5 sublibraries defined by biological categories and includes a negative control (NC) control library and an additional custom test library targetting 86 genes (50 gRNAs per gene).
pTargetF_lac_sacB-1-m - does not express dCas9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Pooled Library||113134||Lib 1 - Essential, stress response, biosynthesis genes†||1||$400||Add to Cart|
|Pooled Library||113135||Lib 2 - Other genes†||1||$400||Add to Cart|
|Pooled Library||113136||Lib 3 - Transport & other enzymatic genes†||1||$400||Add to Cart|
|Pooled Library||113137||Lib 4 - DNA-binding, carbohydrate metabolic, catabolic, RNA-coding genes†||1||$400||Add to Cart|
|Pooled Library||113138||Lib 5 - Promoters, ribosome binding sites†||1||$400||Add to Cart|
|Pooled Library||113146||Lib NC - Non-targeting gRNAs (negative controls)†||1||$350||Add to Cart|
|Pooled Library||113147||Lib Test - Customized library targeting 86 genes†||1||$350||Add to Cart|
If you would like to receive Lib 1 - 4 + NC (113134 - 7 + 113146) in a single order, please email [email protected] for pricing.
|Pooled Library||Genes Targeted||gRNAs|
+ Number of clusters targeted by at least one gRNA; clusters are one single gene or homologous genes with similar sequences.
SpeciesEscherichia coli (E. coli)
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:E. coli Genome-wide Inhibition library was a gift from Chong Zhang (Addgene #)
For your References section:For #113134, #113135, #113136, #113137, #113146: Pooled CRISPR interference screening enables genome-scale functional genomics study in bacteria with superior performance. Wang T, Guan C, Guo J, Liu B, Wu Y, Xie Z, Zhang C, Xing XH. Nat Commun. 2018 Jun 26;9(1):2475. doi: 10.1038/s41467-018-04899-x. PubMed 29946130For #113138, #113147: Improved sgRNA design in bacteria via genome-wide activity profiling. Guo J, Wang T, Guan C, Liu B, Luo C, Xie Z, Zhang C, Xing XH. Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572. PubMed 29982721