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Improved sgRNA design in bacteria via genome-wide activity profiling.
Guo J, Wang T, Guan C, Liu B, Luo C, Xie Z, Zhang C, Xing XH
Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
113138E.coli_GW-sgRNA_Lib5Genome-wide gRNA library for E. coli, sub5
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113147LibTestCustomized sgRNA library targeting 86 E. coli genes
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113150pCas9-J23109Plasmid constitutively expressing Cas9 protein
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113151pdCas9-J23109Plasmid constitutively expressing dCas9 protein
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113152peSpCas9-J23109Plasmid constitutively expressing eSpCas9 protein
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113153peSpdCas9-J23109Plasmid constitutively expressing eSpdCas9 protein
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119701pCas9-J23113Expression of Cas9 with a weak promoter
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