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Addgene

peSpCas9-J23109
(Plasmid #113152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 113152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    N.A.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    eSpCas9
  • Mutation
    K810A & K1003A & R1060A

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    peSpCas9-J23109 was a gift from Chong Zhang (Addgene plasmid # 113152 ; http://n2t.net/addgene:113152 ; RRID:Addgene_113152)

  • For your References section:

    Improved sgRNA design in bacteria via genome-wide activity profiling. Guo J, Wang T, Guan C, Liu B, Luo C, Xie Z, Zhang C, Xing XH. Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572. 10.1093/nar/gky572 PubMed 29982721
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