PurposePlasmid constitutively expressing Cas9 protein
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||113150||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeBacterial Expression
Growth in Bacteria
Copy numberLow Copy
- Cloning method Gibson Cloning
Please visit https://www.biorxiv.org/content/early/2018/02/26/272377 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCas9-J23109 was a gift from Chong Zhang (Addgene plasmid # 113150 ; http://n2t.net/addgene:113150 ; RRID:Addgene_113150)
For your References section:Improved sgRNA design in bacteria via genome-wide activity profiling. Guo J, Wang T, Guan C, Liu B, Luo C, Xie Z, Zhang C, Xing XH. Nucleic Acids Res. 2018 Aug 21;46(14):7052-7069. doi: 10.1093/nar/gky572. 10.1093/nar/gky572 PubMed 29982721