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Purpose
The Murine cell surface protein knockout (McspKO) Library is a CRISPR library targeting 1,157 cell surface genes and 98 control genes, with four sgRNAs per gene.
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Vector Backbone
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Depositing Labs
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | ||
|---|---|---|---|---|---|---|
| Pooled Library | 200445 | McspKO Library | 1 | $ 380 | Add to Cart | |
Library Details
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SpeciesMouse
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Genes targeted1,157
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gRNAs5,001
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Controls98 (49 essential genes, 49 nonessential genes)
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Lentiviral Generation3rd
Library Shipping
This library is delivered as suspended DNA in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaws.
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Volume∼20 µL
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Concentration50 ng/µL
Resource Information
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Protocols
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Depositor Data
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Terms and Licenses
Depositor Comments
See the TKO protocol from the Moffat Lab (document linked above) for details on library amplification, viral packaging, transduction, screening, sequencing, and analysis, including LCV2 primers for amplification. The depositing lab recommends using DrugZ to identify synergistic or suppressor chemical genetic interactions and BAGEL to analyze fold changes and identify essential or non-essential genes.
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
Murine Cell Surface Protein Knockout (McspKO) Library was a gift from Junjie Chen (Addgene #200445) -
For your References section:
Integrated screens uncover a cell surface tumor suppressor gene KIRREL involved in Hippo pathway. Wang C, Feng X, Su D, Chen Z, Wang S, Tang M, Huang M, Nie L, Zhang H, Li S, Yin L, Johnson RL, Hart T, Chen J. Proc Natl Acad Sci U S A. 2022 Jun 21;119(25):e2121779119. doi: 10.1073/pnas.2121779119. PubMed 35704761.