CRiNCL - Human CRISPRi Non-coding Libraries
(Pooled Libraries #86538, #86539, #86540, #86541, #86542, #86543, #86544, #86545, #86546, #86547, #86548, #86549, #86550)
These CRISPRi subpooled libraries (10 gRNAs/TSS) are designed to target long non-coding RNA (lncRNA)-expressing genes rather than coding genes in order to discover non-coding genes with cellular functions.
- Vector Backbone
pCRISPRia-v2 - does not express Cas9
- Depositing Labs
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene # )
For your References section:CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, He D, Attenello FJ, Villalta JE, Cho MY, Chen Y, Mandegar MA, Olvera MP, Gilbert LA, Conklin BR, Chang HY, Weissman JS, Lim DA. Science. 2017 Jan 06. Vol. 355, Issue 6320. pii: aah7111. PubMed 27980086