Pooled Libraries
CRiNCL - Human CRISPRi non-coding libraries

CRiNCL - Human CRISPRi Non-coding Libraries
(Pooled Libraries #86538, #86539, #86540, #86541, #86542, #86543, #86544, #86545, #86546, #86547, #86548, #86549, #86550)

Purpose

These CRISPRi subpooled libraries (10 gRNAs/TSS) are designed to target long non-coding RNA (lncRNA)-expressing genes rather than coding genes in order to discover non-coding genes with cellular functions.
Vector Backbone

pCRISPRia-v2 - does not express Cas9

Depositing Labs

Jonathan Weissman
Publication

Liu et al. Science. 2017 Jan 06. Vol. 355, Issue 6320. pii: aah7111. (How to cite )

Ordering

Item	Catalog #	Description	Quantity
Pooled Library	86538	CRISPRi non-coding library (CRiNCL) - Common to all 7 cell lines^†	1	$600	Add to Cart
Pooled Library	86539	CRISPRi non-coding library (CRiNCL) - Common to all 5 cancer cell lines^†	1	$300	Add to Cart
Pooled Library	86540	CRISPRi non-coding library (CRiNCL) - Unique to iPSC^†	1	$300	Add to Cart
Pooled Library	86541	CRISPRi non-coding library (CRiNCL) - Unique to HFF^†	1	$300	Add to Cart
Pooled Library	86542	CRISPRi non-coding library (CRiNCL) - Unique to U87^†	1	$300	Add to Cart
Pooled Library	86543	CRISPRi non-coding library (CRiNCL) - Unique to HEK293T^†	1	$300	Add to Cart
Pooled Library	86544	CRISPRi non-coding library (CRiNCL) - Unique to K562^†	1	$300	Add to Cart
Pooled Library	86545	CRISPRi non-coding library (CRiNCL) - Unique to HeLa^†	1	$300	Add to Cart
Pooled Library	86546	CRISPRi non-coding library (CRiNCL) - Unique to MCF7^†	1	$300	Add to Cart
Pooled Library	86547	CRISPRi non-coding library (CRiNCL) - U87 & HEK293T^†	1	$300	Add to Cart
Pooled Library	86548	CRISPRi non-coding library (CRiNCL) - K562, HeLa, & MCF7^†	1	$300	Add to Cart
Pooled Library	86549	CRISPRi non-coding library (CRiNCL) - K562 & HeLa^†	1	$300	Add to Cart
Pooled Library	86550	CRISPRi non-coding library (CRiNCL) - iPSC & HFF^†	1	$300	Add to Cart

This material is available to academics and nonprofits only.

^† A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with dCas9-KRAB-BFP (#46911) or otherwise used with cell lines already expressing inhibitory dCas9.
If you would like to receive all thirteen sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library	Genes Targeted	gRNAs	Control gRNAs
86538	1329	13,548	258
86539	675	6903	153
86540	2965	29,976	326
86541	4103	41,511	481
86542	2307	23,317	247
86543	2253	22,869	339
86544	2831	28,501	191
86545	2337	23,693	323
86546	2913	29,617	487
86547	1443	14,694	264
86548	875	8944	194
86549	1051	10,794	284
86550	1269	12,950	260

Species
Human
Lentiviral Generation
3rd

Library Shipping

Each library will be delivered as a pooled DNA library in a microcentrifuge tube on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.

Volume
~20 µL
Concentration
50 ng/µL

Resource Information

Protocols
- Illumina Sequencing Sample Preparation (752 KB)
- Liquid Culture Re-Amplification Protocol (71 KB)
- Cell Line Creation and Validation Primer (125 KB)
- CRISPRi/a Custom Library Cloning Protocol (92 KB)
- For more links and protocols, please see the Weismann lab's CRISPRi/a screening materials page.
Depositor Data
- TSS Annotations (1.4 MB)
- CRiNCL library sgRNAs (18.5 MB)
Terms and Licenses
- UBMTA
- Evrogen Limited Use Label License for FPs

Depositor Comments

The data analysis pipeline for analyzing sequencing data is available on GitHub.

schematic of crincl sublibraries — Schematic of CRiNCL sublibraries

How to cite this pooled library (Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.

Example for your Materials & Methods section:

Human CRISPRi non-coding library (CRinCL) was a gift from Jonathan Weissman (Addgene # )
For your References section:

CRISPRi-based genome-scale identification of functional long noncoding RNA loci in human cells. Liu SJ, Horlbeck MA, Cho SW, Birk HS, Malatesta M, He D, Attenello FJ, Villalta JE, Cho MY, Chen Y, Mandegar MA, Olvera MP, Gilbert LA, Conklin BR, Chang HY, Weissman JS, Lim DA. Science. 2017 Jan 06. Vol. 355, Issue 6320. pii: aah7111. PubMed 27980086

CRiNCL - Human CRISPRi Non-coding Libraries(Pooled Libraries #86538, #86539, #86540, #86541, #86542, #86543, #86544, #86545, #86546, #86547, #86548, #86549, #86550)