Human Genome-wide CRISPRi-v2 Libraries
(Pooled Libraries #83969, #1000000090)
Version 2 - Human genome-scale CRISPRi library for inhibition of gene transcription. Available as 5 gRNAs (single half-library) or 10 gRNAs per gene.
- Vector Backbone
pCRISPRia-v2 - does not express Cas9
- Depositing Labs
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:hCRISPRi-v2 library was a gift from Jonathan Weissman (Addgene ID #)
For your References section:Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.PubMed 27661255