Human Genome-wide CRISPRa-v2 Libraries
(Pooled Libraries #83978, #1000000091)
Version 2 - Human genome-scale CRISPRa library for activation of gene transcription. Available as 5 gRNAs (single half-library) or 10 gRNAs per gene.
pCRISPRia-v2 - does not express Cas9
- #83978: 104,540
- #1000000091: 209,080
- #83978: 1,895
- #1000000091: 3,790
These libraries are delivered as suspended DNA in microcentrifuge tubes on blue ice. The tube's contents will not necessarily be frozen. For best results, minimize freeze-thaw cycles.
Pooled Library #83978 will be delivered as a single tube containing a pooled half-library with 5 gRNAs per gene.
Pooled Library #1000000091 will be delivered as two tubes, each containing a pooled half-library with 5 gRNAs per gene (for a total of 10 unique gRNAs per gene).
Terms and Licenses
These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the pooled libraries were described, and include Addgene in the Materials and Methods of your future publications.
Example for your Materials & Methods section:hCRISPRa-v2 library was a gift from Jonathan Weissman (Addgene ID #)
For your References section:Compact and highly active next-generation libraries for CRISPR-mediated gene repression and activation . Horlbeck MA, Gilbert LA, Villalta JE, Adamson B, Pak RA, Chen Y, Fields AP, Park CY, Corn JE, Kampmann M, Weissman JS Elife. 2016 Sep 23;5. pii: e19760. doi: 10.7554/eLife.19760.PubMed 27661255